She was a relative of former President James Knox Polk.
Huff began her acting career at the age of 15. She toured in stage productions of Ben-Hur and Graustark, and made her motion picture debut in 1913 with In the Bishop's Carriage and Caprice. In 1916 she secured the ingenue role opposite Jack Pickford in the Booth Tarkington comedy Seventeen.
Her later silent films included roles in Great Expectations (1917), The Seventh Day (1922), Disraeli (1921), and Oh, You Women! (1919). She was featured on Broadway in Mary the Third and The New Englander. Huff was featured in motion pictures produced by Famous Players-Lasky and Paramount Pictures, and continued in films until 1922.
Huff was a director of the Friends of the Theater and Music Collection at the Museum of the City of New York.
Biography by Hans J. Wollstein:
A stage ingenue of some importance who had appeared in the original Broadway version of Ben Hur, brunette Louise Huff became a star with the pioneer Lubin Mfg. Company of Philadelphia. In scores of one- and two-reel melodramas and Westerns from the very early 1910s, Huff was especially popular in tandem with Edgar Jones, whom she married. Together, they relocated to California in the mid-1910s but her career was already on the wane and she retired in 1922. Her sister, Justina Huff, was also a Lubin star. She was married to Edwin A. Stillman, who was president of Watson-Stillman, manufacturers of hydraulic machinery. In her later years, she resided at 155 East 72nd Street in New York, and she died in New York's Doctors Hospital in 1973.